Core Technologies

PATENTED Direct Heat, Air™ (DHA) HEATING

The Direct Heat, Air™ (DHA) chamber heating system is an exclusive feature of the SANYO MCO-20AIC, MCO-18AIC, MCO-18M, MCO-5AC, MCO-17AC CO2 incubators. The patented Direct Heat (Air) Air Jacket Heating System eliminates the need for a conventional water jacket, while achieving temperature stability, uniformity and fast recovery following door openings.

• The microprocessor controller directs proportional distribution of electrical power to a series of independent heating sources in the incubator.
• Arranged in three zones, these sources include the side, top and rear walls, the chamber base and the outer door.
• Together, the heating sources maintain accurate temperature control over a range from 5°C above ambient to +50°C, with setpoint accuracy to 0.1°C and uniformity better than 0.25°C throughout the chamber.
• Each zone is controlled according to the demands of the microprocessor which manages continuous feedback from the incubator via a PID (proportional, integral and derivative) algorithm.

Sterisonic GxP Design

Elevated Humidity, Low Water Level Warning
To avoid cell culture desiccation, the SANYO Sterisonic™ GxP CO2 incubator maintains ~95% RH at 37°C.

• Humidification is achieved by a combined forced-air and natural evaporation method enhanced by the Direct Heat and Air Jacket base heater and protected by an optical water level indicator to warn of low water in the removable humidity pan.
• The humidity removes easily for regular cleaning and refill.
• When filled, the pan slides into place and the optical sensor returns to position and the SafeCell™ UV lamp destroys any contaminants introduced during the process.
• For the H202 in situ sterilization cycle the humidity pan is repositioned against the chamber wall to eliminate the need for seperate autoclaving.

MCO-20AIC shown with five shelves, included.

Zone	Location	Energy	Microprocessor Control
Main	Side, top and rear walls	Variable	Energizes any, all or a combination of heating elements as required
Base	Floor	Variable
Front	Outer door	Variable

An air jacket with five independent heating elements arranged in three zones surrounds the interior chamber. The microprocessor control system apportions energy to heaters in response to chamber demand and ambient temperature.

• Side, top and real walls form the dominant radiant heat source.
• The base heater elevates the humidity reservoir water temperature to achieve 95% RH at 37°C
• The outer door heater warms the inner glass in response to ambient conditions to eliminate condensation on the glass and around the opening, and to assure interior uniformity.